Pci neo vector promega

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PROTEIN EXPRESSION PROMEGA NOTES WWW.PROMEGA.COM NUMBER 101 FEBRUARY 2009 9 CONCLUSION Mar 08, 1999 Name: pCI-neo: Description: Mammalian expression vector with CMV promoter, T7 and T3, chimeric intron; amp resistance (neomycin for mammalian selection); restriction enzyme cloning. Promega provides the FuGENE® HD Protocol Database to help identify a protocol for your cell line when using the FuGENE® HD Transfection Reagent. A drop-down menu allows you to search the database by cell line, plate type and number of cells to be transfected. pCI-neo Mammalian Expression Vector (Cat.# E1841)] can be selected for stable vector and the DsRed-Golgi expression vector. After 24 h of transfection, fixation and permeabilization were performed as described above, and CR-1 proteins were stained with 5 g/ml MAB2771 and Alexa Fluor 488-conjugated secondary anti-body.Endoplasmicreticulum(ER)wasvisualizedwithanti-cal-nexin rabbit polyclonal antibody (Santa Cruz, Santa The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells.

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2004 May 21. 304(5674):1164-6. This plasmid is available through Addgene. Belgium). Restriction digestion of NS4B gblock and pCI-neo vector (Promega, Cat# E1841) was performed by using XhoI and XbaI restriction enzymes. Following restriction, NS4B gblock and pCI-neo vector were ligated using T4 DNA ligase (Prome ga). The ligation mix was transformed in electro- Article Snippet: The cDNA coding for FGFR2c was amplified by pMD18T-simple FGFR2c (HG10824-M, Sino Biological Inc., Beijing, China) and subcloned in pCI-neo expression vector using standard procedures (Promega, Madison, WI) and the resulting pCI-neo FGFR2c construct was verified by sequencing.

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells.

Manufacturer: Promega™ E1841 View more versions of this product pCI-neo Mammalian Expression Vector. 恒常的なタンパク質発現のための哺乳動物用発現ベクターでネオマイシン耐性遺伝子により安定発現株を選別. E1841.

Contains a neomycin resistance gene and the CMV immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. Transfected cells can be selected with G-418.In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors

恒常的なタンパク質発現のための哺乳動物用発現ベクターでネオマイシン耐性遺伝子により安定発現株を選別. E1841. pAdVAntage™ Vector.

Many Promega Vectors are reporter vectors containing various combinations of luciferase genes (firefly, Renilla, NanoLuc luciferase) with other genetic elements. The luminescence signal from these reporter vectors is used as an indicator of the activity of any gene/DNA element fused to the reporter. pCI-neo Mammalian cell expression vector with the CMV promoter and a neomycin (G418) resistance marker. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer.

The pCI-neo Vector can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G-418. Return to this vector's summary. LOCUS CVU47120 5474 bp DNA circular SYN 20-FEB-1996 DEFINITION Cloning vector pCI-neo, mammalian expression vector… pCI-neo Mammalian Expression Vector. DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression.

Transfected cells can be selected with G-418.In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors Promega pCI and pCI-neo Mammalian Expression Vectors pCI-neo Mammalian Expression; 20μg Contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in mammalian cells. The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a … This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Multiple cloning sites exist for easy insertion of cDNA.

LOCUS CVU47120 5474 bp DNA circular SYN 20-FEB-1996 DEFINITION Cloning vector pCI-neo, mammalian expression vector… pCI-neo Mammalian Expression Vector. DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression. E1841. pAdVAntage™ Vector. When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation. E1711 pCI 4,006 Y T7 N Y Y T7 EEV pCI-neo 5,472 Y T7, T3 N Y Y T3, T7 EEV Key: S - SP6 M - M13 *Note: The T7 EEV (Eukaryotic Expression Vector) Promoter Primer (Cat.# Q6700) is designed specifically for use with Promega’s mammalian expression vectors. pCI-neo-ER vector was constructed by insertion of human ER cDNA derived from pIC-ER-F (ATCC, Manassas, Virginia, U.S.A.) in pCI-neo mammalian expression and neomycin resistant vector (Promega, Madison, Wisconsin, U.S.A.).

A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression.

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The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression.

pCMVT n T™ Vector contains a CMV enhancer/promoter region, which enables strong constitutive expression in many cell types, while RNA phage promoters enable RNA synthesis in vitro. pCI-neo Mammalian Expression Vector. DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression.